Single-cell analysis has become one of the most important applications of microfluidic technology in recent years. It refers to the process of isolation, manipulation, and analysis of the cells at single-cell resolution in microfluidic chips. Depending on the application, single-cell analysis chips can include any...
L-form bacteria also known as cell wall deficient (CWD) bacteria are strains with no cell wall developed from initial walled bacteria. Cell walls play an important during the division of the cells. However, since L-form lacks a cell wall, the division is disorganized and daughter...
The majority of microfluidic devices for Assisted Reproductive Technologies (ART) have aimed at enhancing sperm selection and analysis so far. A novel microfluidic chip, however, turns the page by offering a more efficient protocol for oocyte cryopreservation. The design rationale and performance of this microfluidic...
Microfluidic single-cell analysis platforms often take advantage of droplet microfluidic for encapsulating the cells within nL-pL droplets at high throughputs. Depending on the target outcome (genomics, epigenomics, transcriptomics, etc.), specific protocols are available for the tests.Cells in a culture can be highly heterogeneous and impose...
Undoubtedly, microfluidics has opened many new windows of opportunities for single-cell analysis. Droplet microfluidics, for example, has shown great promises for encapsulation, manipulation, and incubation of single-cells within nL-pL droplets at very high throughputs. As the field grows more and more methods get developed providing...