Screening for microbial proteolytic activity is essential in various biotechnological applications, including bioenergy, food processing, and pharmaceuticals. Traditional methods, such as cultivation on agar plates with skimmed milk, often result in low throughput and potential false positives from non-proteolytic activities. The need for more accurate,...
In droplet microfluidics, high-throughput screening is critical for analyzing large cellular or molecular libraries at single-cell resolution. However, achieving high sorting accuracy remains a challenge, especially with the droplet size variability inherent in multi-step assays or extended incubations. Conventional microfluidic sorting methods, such as those...
Microswimmers are tiny robotic entities designed to move through fluids, and they hold significant promise for applications such as targeted drug delivery and minimally invasive surgery. Recent advancements in micro-robotics have paved the way for significant developments in biomedical applications. Among these, the creation of...
In the continuous search to understand life's origins and harness biotechnology's potential, scientists have made a new discovery using high-throughput droplet microfluidics: a minimalist enzyme, derived from a library of de novo designed proteins, exhibiting remarkable biological activity. This enzyme, named mini-cAMPase, challenges our understanding...
The ability to analyze individual cells allows scientists to uncover a wealth of information hidden in the cellular heterogeneity of tissues, leading to breakthroughs in understanding diseases and developing targeted treatments. However, despite the critical importance of this research, traditional methods of single-cell analysis, particularly...