Biological systems are regulated through networks of highly complex behaviors. Bottom-up synthetic biology provides a means for deconvoluting the underlying architecture governing these network-based behaviours. Droplet microfluidics has been the gold standard for creating complex synthetic cells from the bottom-up. To better design such systems,...
Abstract "Infrared (IR) plasmonic nanoantennas (PNAs) are powerful tools to identify molecules by the IR fingerprint absorption from plasmon-molecules interaction. However, the sensitivity and bandwidth of PNAs are limited by the small overlap between molecules and sensing hotspots and the sharp plasmonic resonance peaks. In addition...
Fluid flow through a porous media can be seen in many biological and geological systems. The pores in porous media induce very complex fluid patterns that are sometimes overlooked. For example, the complex morphology in these systems is often associated with dead-ends resulting in a...
Abstract "Most methods for single-cell transcriptome sequencing amplify the termini of polyadenylated transcripts, capturing only a small fraction of the total cellular transcriptome. This precludes the detection of many long non-coding, short non-coding and non-polyadenylated protein-coding transcripts and hinders alternative splicing analysis. We, therefore, developed VASA-seq...
Technological advancements in sequencing technologies, as well as high-throughput methods such as droplet microfluidics, have led to more widespread use of transcriptomics in single-cell research. However, not all single-cell omics are equally advanced. For instance, although proteins can be of utmost importance in disease diagnostics,...