10 Nov Microfluidic analysis of cell size fluctuation in starving bacteria
“In stable environments, cell size fluctuations are thought to be governed by simple physical principles, as suggested by recent findings of scaling properties. Here, by developing a microfluidic device and using E. coli, we investigate the response of cell size fluctuations against starvation. By abruptly switching to non-nutritious medium, we find that the cell size distribution changes but satisfies scale invariance: the rescaled distribution is kept unchanged and determined by the growth condition before starvation. These findings are underpinned by a model based on cell growth and cell cycle. Further, we numerically determine the range of validity of the scale invariance over various characteristic times of the starvation process, and find the violation of the scale invariance for slow starvation. Our results, combined with theoretical arguments, suggest the relevance of the multifork replication, which helps retaining information of cell cycle states and may thus result in the scale invariance.”
“a Entire view of the device. Microwells are created on a glass coverslip. We attach a polydimethylsiloxane (PDMS) pad on the coverslip with a square frame seal to fill the system with liquid medium. b Cross-sectional view inside the PDMS pad. A polyethylene terephthalate (PET)–cellulose bilayer porous membrane is attached via the biotin–streptavidin bonding. Note that there are two outlets as in (a).” Reproduced underCreative Commons Attribution 4.0 International License from Shimaya, T., Okura, R., Wakamoto, Y. et al. Scale invariance of cell size fluctuations in starving bacteria. Commun Phys 4, 238 (2021).
Figures and the abstract are reproduced from Shimaya, T., Okura, R., Wakamoto, Y. et al. Scale invariance of cell size fluctuations in starving bacteria. Commun Phys 4, 238 (2021). under Creative Commons Attribution 4.0 International License
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