Abstract "Most methods for single-cell transcriptome sequencing amplify the termini of polyadenylated transcripts, capturing only a small fraction of the total cellular transcriptome. This precludes the detection of many long non-coding, short non-coding and non-polyadenylated protein-coding transcripts and hinders alternative splicing analysis. We, therefore, developed VASA-seq...
Technological advancements in sequencing technologies, as well as high-throughput methods such as droplet microfluidics, have led to more widespread use of transcriptomics in single-cell research. However, not all single-cell omics are equally advanced. For instance, although proteins can be of utmost importance in disease diagnostics,...
Abstract "The cytoskeleton is an essential component of a cell. It controls the cell shape, establishes the internal organization, and performs vital biological functions. Building synthetic cytoskeletons that mimic key features of their natural counterparts delineates a crucial step towards synthetic cells assembled from the bottom...
It is well known that the presence of mechanical cues is of significant importance in developmental biology. The extracellular forces that the cells experience in space where gravity is negligible compared to the earth differ from their natural condition. This change in the gravitational field...
Abstract "Extremely rare circulating tumor cell (CTC) clusters are both increasingly appreciated as highly metastatic precursors and virtually unexplored. Technologies are primarily designed to detect single CTCs and often fail to account for the fragility of clusters or to leverage cluster-specific markers for higher sensitivity. Meanwhile,...