Microfluidic single-cell analysis platforms often take advantage of droplet microfluidic for encapsulating the cells within nL-pL droplets at high throughputs. Depending on the target outcome (genomics, epigenomics, transcriptomics, etc.), specific protocols are available for the tests.Cells in a culture can be highly heterogeneous and impose...
Undoubtedly, microfluidics has opened many new windows of opportunities for single-cell analysis. Droplet microfluidics, for example, has shown great promises for encapsulation, manipulation, and incubation of single-cells within nL-pL droplets at very high throughputs. As the field grows more and more methods get developed providing...
It was only less than 100 years ago that Sir Hans Adolf Krebs measured the metabolism rate of muscle cells by exposing fresh pigeon breast meat to different reagents to discover the Krebs cycle (also known as citric acid cycle or tricarboxylic acid cycle or...
Extracellular matrix (ECM) is the non-cellular element in the body that provides scaffolding as well as biochemical and biomechanical cues for the cells. Synthetic and natural ECMs are widely used in tissue and organ engineering applications. Although native tissues are often inhomogenous, many of the...
A group of researchers has now taken organ on a chips to a new level by developing a multiorgan system with circulating immune cells to mimic the immunosurveillance process. The results of this study that hinged upon a three organ microfluidic chip are reported in...